Figure 3.

Abhydrolase domain of NSL3 is required for its binding activity to OGT1. A, enhancement of binding activity between abhydrolase domain-containing N-terminal NSL3 and N-terminal OGT1-1. 293T cells were co-transfected with N-terminal OGT1–1 and full-length NSL3 or a deletion mutant of NSL3 (NSL3dC) (upper panel), and bound proteins were detected using Western blotting with the indicated antibodies. B, reduction of binding activity between NSL3 without the abhydrolase domain (NSLdD) and OGT1. Similarly, 293T cells were co-transfected with OGT1 and abhydrolase domain-deleted NSL3 (upper panel), and bound proteins were measured by Western blotting with anti-FLAG or anti-Myc antibody. IB, immunoblot; Fl, FLAG.