Figure 3.

Gwl associates with PP1 in a cell cycle-dependent manner. A, MBP-PP1γ pulldown was performed in interphase (Int) or CSF egg extracts. The pulldown product, extract input, and a control (Ctr) pulldown were immunoblotted for Gwl and MBP. B, immunoprecipitation of PP1γ was performed in interphase or CSF egg extracts and immunoblotted for Gwl and PP1γ. C, immunoprecipitation of PP1γ was performed in interphase or M phase HeLa cell lysates and immunoblotted for human Gwl and PP1γ. HeLa cells were synchronized in interphase or M phase by thymidine block or nocodazole arrest, respectively. The data in this figure are representative of three or more independent experiments.