Figure 2.

Oxidation kinetics of the reduced mitoNEET [2Fe-2S] clusters by oxygen. MitoNEET (containing 10 μm [2Fe-2S] clusters) was incubated with FMN (0.1 μm) and NADH (20 μm) under aerobic conditions. Fre (0.1 μm) was then added to the incubation solution to initiate the reaction. The amount of NADH in the incubation solution was measured from the absorption peak at 340 nm. The amount of the reduced mitoNEET [2Fe-2S] clusters was measured from the different absorption at absorption peaks of 455 nm and 420 nm. The amounts of NADH and the reduced mitoNEET [2Fe-2S] clusters in the incubation solution were plotted as a function of incubation time. The data are representative of three independent experiments.