Figure 2.
HMGCS1 controls production of ketone body acetoacetate to selectively promote cell proliferation of BRAFV600E-expressing melanoma cells. A–D, intracellular concentrations of acetoacetate (AA) (A and B) and rescue effect of adding acetoacetate in culture media on cell proliferation rates (C and D) were examined in melanoma (A and C) and colon cancer (B and D) cells upon HMGCS1 knockdown. Mean ± S.D. Cell proliferation rates were determined by daily cell counting. Error bars indicate ± S.D.; n = 3 each. p values were obtained by two-tailed Student's t test. Western blot shows knockdown efficiency of HMGCS1 in corresponding cells.