Figure 3.
HMGCS1 is selectively important to regulate acetoacetate that specifically promotes BRAFV600E-MEK1 binding and consequent MEK-ERK activation in BRAFV600E-expressing cells. A–D, effect of adding acetoacetate in culture media on phosphorylation levels of MEK1 and ERK1/2 (A and B) and BRAF-MEK1 interaction (C and D) in distinct melanoma (A and C) and colon cancer (B and D) cells and cells with stable HMGCS1 knockdown. IP, immunoprecipitate. Quantification error bars along with each Western blot indicate ± S.D.; p values were obtained by two-tailed Student's t test. Western blots are from a representative experiment for which n = 3 for A, B, and D and n = 4 for C.