Figure 5.
HMGCS1 co-localizes with HMGCL and BRAFV600E in cytosol and regulates HMGCL protein stability. A, subcellular localization of HMGCS1, BRAF, and HMGCL in cytoplasm, nucleus, and mitochondria in BRAF WT cells HMCB and BRAFV600E-positive cells A2058 and A375. Actin, PARP, and TOM40 were included as markers for cytoplasm, nucleus, and mitochondria, respectively. B and C, effect of stable knockdown of HMGCL (B) or HMGCS1 (C) on HMGCS1 or HMGCL protein expression (left panels) and mRNA levels (right panels), respectively. D, results of Western blot showing effect of cycloheximide (CHX) treatment on HMCGL1 in the melanoma cells in the presence or absence of stable knockdown of HMGCS1.