Figure 7.
HMGCS1 is specifically important for tumor growth potential of BRAFV600E-expressing melanoma cells. A and B, growth rates, masses, and sizes of tumors harvested from xenograft mice injected with NRAS Q61K-expressing HMCB cells (A) or BRAFV600E-positive A375 cells (B) harboring an empty vector or HMGCS1 shRNA. Error bars indicate ± S.D. p values of tumor size and weight were obtained by two-way ANOVA. C and D, phosphorylation levels of MEK1 and ERK1/2 assessed by Western blot (C) and relative acetoacetate levels (D) in tumors obtained from xenograft mice injected with control or HMGCS1 knockdown HMCB or A375 cells. p values of results detecting acetoacetate concentrations were obtained by two-tailed Student's t test. Error bars indicate ± S.D.; n = 3 each.