Figure 11.

Single-round viral infectivity assay in TZM-bl cells. Comparison of infectivity for full-length viral clones containing WT or differentially glycosylated JRFL gp160 Env using the TZM-bl assay. This assay gives the readout of infectivity as relative luminescence units (RLUs). The relative amount of virus in cell supernatants was normalized following a p24 assay, and an equal amount of each virus was used for the infectivity assay. Mutant viruses lacking either all outer domain glycans (ΔG13 gp160) or all core gp120 glycans (ΔG13 gp160) retained infectivity comparable with that of viruses having WT gp160 Env. Error bars represent standard deviation for the data from two independent experiments.