Table 2.
KD values for the binding of various OD fragments displayed on the yeast surface to VRC01 antibody, determined by FACS titrations
KD indicates equilibrium dissociation constant; S.D. is standard deviation for the data from two independent experiments.
| Construct name | KD ±S.D. |
|---|---|
| nm | |
| ODYCO (fully glycosylated) | >300–400 |
| ΔG4-ODYCO (partially glycan-free) | 27 ± 3 |
| ΔG14-ODYCO (completely glycan-free) | 10 ± 1 |
| ΔG14-ODYCO-D368Ra (completely glycan-free negative control) | >700 |
| WT core gp120 (positive control) | 24.3 ± 0.4 |
a D368R mutation is known to decrease the binding of gp120 with CD4-binding site antibodies such as VRC01.