Table 4.
Nomenclature and description of HIV-1 gp120 constructs based on JRFL strain
| No. | Construct name | Description |
|---|---|---|
| 1 | WT core gp120 | Wild-type JRFL gp120 lacking V1/V2 and V3 loops. It contains residues 31–127-GAG-195–297-GAG-330–507 |
| 2 | ΔG15 core gp120 | Core gp120 devoid of all 15 PNGS |
| 3 | ΔG4 core gp120 | ΔG4 core gp120 contains all PNGS except for four sites (at residues 276, 386, 392 and 463) near the CD4-binding region |
| 4 | ΔG11 core gp120 | This construct contains only four PNGS: two in the inner domain of gp120 (at residues 88 and 241) and two at the interface of the inner and outer domains of gp120 (at residues 262 and 276) |
| 5 | ΔG12a core gp120 | An additional PNGS (N88Q) removed in ΔG11 core gp120 background, now contains three PNGS |
| 6 | ΔG12b core gp120 | An additional PNGS (N241K) removed in ΔG11 core gp120 background now contains three PNGS |
| 7 | ΔG12c core gp120 | An additional PNGS (N262D) removed in ΔG11 core gp120 background now contains three PNGS |
| 8 | ΔG12d core gp120 | An additional PNGS (N276D) removed in ΔG11 core gp120 background now contains three PNGS |
| 9 | ΔG13 core gp120 | Contains only two PNGS at inner domain residues 88 and 241 |
| 10 | ΔG14a core gp120 | Contains only one PNGS at residue inner domain residue 241. Mutation N88G at inner domain position 88 in this construct was selected using yeast library screening |
| 11 | ΔG14b core gp120 | Contains only one PNGS at inner domain residue 88. Mutation N241K at inner domain position 241 in this construct was selected using yeast library screening |
| 12 | ΔG15F core gp120 | Devoid of all 15 core gp120 PNGS. Mutations at inner domain positions 88 and 241 in this construct were selected using yeast library screening |
| 13 | E168K ΔG4 full-length gp120 | A ΔG4 core gp120 variant that contains the V1/V2 and V3 loop regions and the E168K mutation in the V1 loop in order to introduce PG9 antibody epitope |