1 |
WT core gp120 |
Wild-type JRFL gp120 lacking V1/V2 and V3 loops. It contains residues 31–127-GAG-195–297-GAG-330–507 |
2 |
ΔG15 core gp120 |
Core gp120 devoid of all 15 PNGS |
3 |
ΔG4 core gp120 |
ΔG4 core gp120 contains all PNGS except for four sites (at residues 276, 386, 392 and 463) near the CD4-binding region |
4 |
ΔG11 core gp120 |
This construct contains only four PNGS: two in the inner domain of gp120 (at residues 88 and 241) and two at the interface of the inner and outer domains of gp120 (at residues 262 and 276) |
5 |
ΔG12a core gp120 |
An additional PNGS (N88Q) removed in ΔG11 core gp120 background, now contains three PNGS |
6 |
ΔG12b core gp120 |
An additional PNGS (N241K) removed in ΔG11 core gp120 background now contains three PNGS |
7 |
ΔG12c core gp120 |
An additional PNGS (N262D) removed in ΔG11 core gp120 background now contains three PNGS |
8 |
ΔG12d core gp120 |
An additional PNGS (N276D) removed in ΔG11 core gp120 background now contains three PNGS |
9 |
ΔG13 core gp120 |
Contains only two PNGS at inner domain residues 88 and 241 |
10 |
ΔG14a core gp120 |
Contains only one PNGS at residue inner domain residue 241. Mutation N88G at inner domain position 88 in this construct was selected using yeast library screening |
11 |
ΔG14b core gp120 |
Contains only one PNGS at inner domain residue 88. Mutation N241K at inner domain position 241 in this construct was selected using yeast library screening |
12 |
ΔG15F core gp120 |
Devoid of all 15 core gp120 PNGS. Mutations at inner domain positions 88 and 241 in this construct were selected using yeast library screening |
13 |
E168K ΔG4 full-length gp120 |
A ΔG4 core gp120 variant that contains the V1/V2 and V3 loop regions and the E168K mutation in the V1 loop in order to introduce PG9 antibody epitope |