Figure 5.

Plasmids expressing V. fischeri genes complemented growth of V. fischeri knock-out mutants. Each panel shows the growth curves for a V. fischeri mutant complemented with an empty vector or vector expressing the deleted gene. The gene listed at the top of each panel indicates the gene deleted in the mutant and expressed in the complementation plasmid. Each solid line represents A₆₀₀ over time, and the associated colored transparent shade indicates the range of standard deviations across biological replicates (n = 3). The medium used in each panel was the DMM minimal medium supplemented with (a) 20 mm glucose, (b) 20 mm mannitol, or (c) 20 mm N-acetylneuraminate. Blue lines represent growth of V. fischeri ES114 with an empty pVSV105 vector. Green lines represent growth of the V. fischeri knock-out mutants (ΔVF_0892, ΔVF_A0062, and ΔVF_0668) with an empty pVSV105 vector. Yellow lines represent growth of V. fischeri knock-out mutants carrying a pVSV105 plasmid expressing the corresponding V. fischeri gene (e.g. ΔVF_0892 + pVF0892).