Figure 6.

Kinetic characterization and substrate specificity of the E. coli PanD and the VF_0892 (PanP) enzymes. Average reaction rates (ΔAU units/min) are shown for three technical replicates of the DC-PEPC-MDH-linked assays performed at the specified aspartate or glutamate concentration with standard deviations shown as the error bars. a, reactions rates with the VF_0892 enzyme at various concentrations of aspartate at either 28 °C (gray) or 37 °C (blue). Solid lines show the nonlinear fitting to the Michaelis-Menten equation using KaleidaGraph. b, reaction rates of PanD at various concentrations of aspartate at 37 °C. c, reaction rates of PanD with a concentration of 8 mm of either glutamate or aspartate and reaction rates of the VF_0892 enzyme with a concentration of 9 mm of either glutamate or aspartate at either 28 or 37 °C.