Figure 2.

EV71 inhibited induction of ISGs stimulated by IFN-β. HeLa cells were infected with EV71 or inoculated with UV-inactivated EV71 for 24 h, followed by treatment with or without IFN-β at 20 ng/ml for another 15 h. A, EV71 infection inhibited induction of ISGs stimulated by IFN-β. Total RNA was prepared from the cells for cDNA synthesis, and fold changes of the ISG gene transcripts were measured by real-time PCR assay. B, inactivated EV71 failed to inhibit induction of ISGs stimulated by IFN-β. Total RNA was prepared from the cells inoculated with inactivated EV71 and subjected to measurement of fold change of ISGs stimulated by IFN-β. C, EV71 inhibited the induction of MX1 and MX2. The cell lysates were prepared from infected or uninfected cells, stimulated with or without IFN-β, and subjected to Western blot analyses with anti-MX1 or anti-MX2 antibodies. The experiments were repeated at least three times. *, p < 0.05 (Student's t test); MW, molecular mass.