Figure 6.

EV71 infection disrupted the interaction of STAT1 and KPNA1 complex and induced KPNA1 degradation. A, the IFN-β-induced complex of STAT/KPNA1 was disrupted. HeLa cells were infected EV71 for 24 h and afterward were treated with IFN-β for 30 min. The cell lysates were prepared and immunoprecipitated with an anti-KPNA1 antibody. The immunoprecipitates were analyzed by SDS-PAGE and Western blot analyses with anti-p-STAT1 antibodies. The inputs of p-STAT1, KPNA1, and β-actin were shown by Western blot analyses with specific antibodies. B, KPNA1 was degraded in EV71 infected RD cells. RD cells were infected with EV71 at an m.o.i. of 2. The cell lysates were prepared at indicated time points for Western blot analysis with antibodies specific for KPNA1. C and D, KPNA1 was degraded in Vero but not in HT-29 cells. Vero (C) and HT-29 (D) cells were infected with EV71, and cell lysates were prepared for Western blot analysis with antibodies specific for KPNA1, TRIF, IRNAR1, and JAK1. ctl, control; IB, immunoblot; IP, immunoprecipitation; MW, molecular mass.