Figure 4.

Involvement of the C/EBP-BS within the hepcidin promoter in IL-1β-induced hepcidin transcription. A, C, and D, HepG2 cells were transfected with tk-Renilla-luc and the indicated reporters and. At 4 h post-transfection, cells were treated with or without IL-1β (10 ng/ml) for 12 h. Firefly luciferase activity normalized to Renilla luciferase activity was calculated, and the relative luciferase activity in untreated cells transfected with hepcidin(−2018)-luc was defined as 1. The data are presented as the mean ± S.E. (n = 3). B, nucleotide sequence of the hepcidin promoter. The putative C/EBP-BS is boxed, and the BMP-REs and STAT-BS are underlined with solid lines and a dotted line, respectively.