Skip to main content
. 2017 Apr 24;292(24):10275–10287. doi: 10.1074/jbc.M116.770974

Figure 9.

Figure 9.

IL-1β expression in macrophages and hepatocytes. A, RAW264. 7 cells were treated with or without LPS (100 ng/ml) for the indicated time. B and C, conditioned media from RAW264.7 cells treated with (CM-LPS) or without (CM-C) LPS (100 ng/ml) for 30 h were prepared as described under “Experimental procedures.” B, an equal volume of the conditioned media was subjected to Western blot analysis to detect IL-1β. HepG2 cells were treated with the conditioned medium from RAW264.7 cells for 24 h, and the expression of IL-1β was examined by RT-qPCR analysis. The levels in the control cells prior to IL-1β treatment (A) or in cells treated with control conditioned medium from RAW264.7 cells (CM-C) (C) were defined as 1. The data are presented as the mean ± S.E. (n = 3). **, p < 0.05 versus cells treated without IL-1β at the respective time points (A) or cells treated with control conditioned medium from RAW264.7 cells (C).