Figure 5.

Malonate-induced removal of oxaloacetate bound to complex II enhances succinate-driven H₂O₂ production by AA5-inhibited permeabilized mitochondria. Permeabilized mitochondria were incubated with (blue traces) and without 1 mm malonate (Malon) (red traces), and the inhibitor was then removed by washing and centrifugation. The resuspended pellet was used for stimulation of ROS production by adding succinate (Succ) (50 μm) to AA5 (1 μm)-inhibited mitochondria (Mito). Dual traces are superimposed for each condition.