Figure 6.

Concentration dependence of fumarate-induced inhibition of H₂O₂ production by complex II-inhibited mitochondria. A, mitochondria (Mito) were incubated with AA5 (1 μm) and fumarate (Fum) at the concentrations indicated at the end of each trace. After permeabilization with alamethicin (Ala), H₂O₂ production progressively decreased with higher [fumarate]. As in other figures, additions to all traces are indicated by black arrows; additions to one trace only are indicated by the arrows of the same color as the trace. B, summary data for inhibition of H₂O₂ production as a function [fumarate], showing half-maximal inhibition at 11 μm. Shaded areas indicate 95% confidence intervals.