Figure 2.

TAP of SF-R7BP and interacting proteins from mouse brain. A, assessing nonspecific background in TAP experiments. TAP was performed as described under “Experimental procedures” using detergent-solubilized brain homogenates from either three SF-R7BP-positive or three SF-R7BP-negative male mice as indicated. The final eluates were separated by SDS-PAGE, and proteins were visualized by SYPRO Ruby staining. B, Western blot (WB) analysis of SF-R7BP, Gβ₅, and RGS7 for each step of the TAP protocol. C, SYPRO Ruby-stained gel of the TAP eluate from three SF-R7BP-positive mouse brains. Arrows indicate regions of the gel that were excised and analyzed by LC-MS/MS. Mass spectrometry data summarized in Table 1 and supplemental Table 1 are organized by gel slice numbers indicated in this panel.