Figure 6.

RH-RhoGEFs enhance interaction between Gα₁₃ and R7-RGS heterotrimers. A, split-luciferase complementation between Gα₁₃(Q/L)-CBGN and CBGC-FLAG-Gβ₅ co-expressed with HA-RGS7 and FLAG-R7BP with or without Myc-LARG, His-Myc-PDZ-RhoGEF, or Myc-p115RhoGEF. Each box represents a data point from one of four independent experiments. B, representative Myc Western blot (WB) showing Myc-LARG, His-Myc-PDZ-RhoGEF, and Myc-p115RhoGEF protein expression levels for conditions used in A. Error bars indicate standard deviation of the mean. Statistical significance was determined using two-tailed, two-sample Student's t test assuming equal variance as deemed appropriate by F-test analysis. **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.