Table 1.
Subset of proteins identified by LC-MS/MS analysis of TAP fractions
Protein bands marked with an arrow in Fig. 2C were analyzed by LC-MS/MS to identify proteins that co-purified with SF-R7BP from transgenic mouse brain. Peptide identifications were accepted if they could be established at greater than 80% probability by the Scaffold local false discovery rate algorithm. All proteins shown here have at least a 99% protein identification (ID) probability as determined using the Protein Prophet algorithm and at least two exclusive unique peptides assigned. Tabulated are protein identification information for R7BP (Rgs7bp protein); R7-RGS family members; and Gβ5, Gαo, and a novel interacting protein, Gα13. See supplemental Table 1 for a complete list of all proteins identified and peptide sequence information.
| Gel slice | Protein name | Accession number | Protein ID probability | Exclusive unique peptides | Exclusive unique spectra | Total spectra | Sequence coverage |
|---|---|---|---|---|---|---|---|
| % | % | ||||||
| 6 | Rgs7bp protein (Mus musculus) | gi 111600355 | 100.00 | 3 | 4 | 12 | 19.50 |
| 7 | Rgs7bp protein (M. musculus) | gi 111600355 | 100.00 | 3 | 4 | 87 | 13.60 |
| 8 | Rgs7bp protein (M. musculus) | gi 111600355 | 100.00 | 2 | 3 | 73 | 22.60 |
| 2 | Regulator of G-protein signaling 9 isoform 1 (M. musculus) | gi 146134422 | 100.00 | 5 | 7 | 21 | 24.30 |
| 3 | Regulator of G-protein signaling 9 isoform 1 (M. musculus) | gi 146134422 | 100.00 | 4 | 4 | 14 | 18.70 |
| 4 | Regulator of G-protein signaling 6 (M. musculus) | gi 29789076 | 100.00 | 6 | 8 | 15 | 20.30 |
| 5 | Regulator of G-protein signaling 6 (M. musculus) | gi 29789076 | 100.00 | 7 | 9 | 11 | 18.60 |
| 4 | Regulator of G-protein signaling 7 (M. musculus) | gi 312222673 | 100.00 | 9 | 11 | 26 | 21.70 |
| 5 | Regulator of G-protein signaling 7 (M. musculus) | gi 312222673 | 99.90 | 5 | 6 | 12 | 12.30 |
| 6 | Guanine nucleotide-binding protein subunit β5 isoform 2 (M. musculus) | gi 41281679 | 100.00 | 4 | 5 | 5 | 5.67 |
| 7 | Guanine nucleotide-binding protein subunit β5 isoform 2 (M. musculus) | gi 41281679 | 100.00 | 17 | 20 | 25 | 26.10 |
| 8 | Guanine nucleotide-binding protein subunit β5 isoform 2 (M. musculus) | gi 41281679 | 100.00 | 5 | 6 | 6 | 8.22 |
| 9 | Guanine nucleotide-binding protein subunit β5 isoform 2 (M. musculus) | gi 41281679 | 100.00 | 3 | 4 | 4 | 5.67 |
| 10 | Guanine nucleotide-binding protein subunit β5 isoform 2 (M. musculus) | gi 41281679 | 100.00 | 2 | 3 | 3 | 5.10 |
| 7 | Guanine nucleotide-binding protein subunit α13 (M. musculus) | gi 89001109 | 100.00 | 2 | 2 | 3 | 10.10 |
| 7 | Guanine nucleotide-binding protein Go subunit α isoform B (M. musculus) | gi 164607137 | 99.00 | 2 | 2 | 3 | 7.63 |