Figure 7.

Domain swapping of conserved region in HSPB1 and HSPB6. A, schematic of the constructs used. The figures were created using DOG (55). B, analytical SEC profiles of an equimolar mixture of the various HSPB1 and HSPB6 swaps following overnight incubation at 37 °C. The control sample (stored at 4 °C) is shown as a black dashed line, and the hetero-oligomer (incubated at 37 °C) is shown as a continuous black line. For each chromatogram, the hetero-oligomer for WT HSPB1 and HSPB6 is shown as a red dashed line. C, non-reducing SDS-PAGE analysis of the complexes between the HSPB1 and HSPB6* swaps. D, control experiment showing the non-reducing SDS-PAGE analysis of different constructs following extensive dialysis to remove DTT.