Table 1.
SAXS analysis of the hetero-oligomeric complexes of HSPB1 and HSPB6 deletion constructs
The SAXS frames were processed using AUTORG and GNOM, both part of the ATSAS package (56). The 2nd to last column shows the Rg value at the elution peak. The last column shows the Rg range corresponding to the frames for which the quality measure in AUTORG was 50% or higher.
| Protein | Estimated average mass based on Porod volume | Calculated average number of subunits | Peak Rg | Rg range |
|---|---|---|---|---|
| (kDa) | Å | |||
| B1.WT | 647.6 | 28.4 | 58.2 | 63.0–56.0 |
| B6.WT | 45.9 | 2.7 | 32.4 | 32.9–27.5 |
| B1 + B6 | 342.4 | 17.2 | 51.1 | 52.4–39.5 |
| B1 + B6.ΔN11 | 311.7 | 16.1 | 49.6 | 50.9–39.7 |
| B1 + B6.Δ11–20 | 212.1 | 10.9 | 48.0 | 51.1–35.2 |
| B1 + B6.Δ21–30 | 490.8 | 25.2 | 56.9 | 57.1–31.5 |
| B1 + B6.Δ31–40 | 478.9 | 24.7 | 57.0 | 57.3–33.2 |
| B1 + B6.Δ41–50 | 356.4 | 18.3 | 52.2 | 52.9–44.9 |
| B1 + B6.Δ51–60 | 134.7 | 6.9 | 41.6 | 48.2–33.7 |
| B1 + B6.Δ61–70 | 337.6 | 17.3 | 51.2 | 51.8–42.5 |