Figure 3.

PKA did not mediate the β₂AR calcium response. a, 1 min of 8-Br-cAMP treatment did not mimic ISO response. b, 30 min of 8-Br-cAMP treatment did not mimic ISO response. c, 1 min of ISO significantly raised pCREB levels (**, p = 0.0065), whereas 1 min of 8-Br-cAMP treatment did not significantly raise pCREB levels (NS). d, 30 min of ISO (**, p = 0.0056) and 8-Br-cAMP treatment (*, p = 0.0133) significantly elevated intracellular pCREB. e, treatment with PKA inhibitors KT-5720 and H-89 for 1 h did not suppress signaling with ISO. Ca²⁺ traces represent three or more independent experiments, and error bars indicate internal replicate S.E. pCREB/CREB graphs are the averages of three independent experiments; error bars indicate S.E. Full blot images are shown in supplemental Fig. S1. AU, absorbance units.