Figure 6.

Supporting evidence against Gα_q coupling to β₂AR in TRPC4 β-expressing HEK-293 cells. Treatment with ISO did not mimic the membrane potential response of the Gα_q-coupled M₃ muscarinic receptors activated with carbachol. Ca²⁺ traces represent three or more independent experiments, and error bars indicate internal replicate S.E. Fig. 4 demonstrates robust membrane potential changes in response to μOR stimulation with DAMGO under these conditions without PTX treatment and robust Ca²⁺ release in response to isoproterenol stimulation under these conditions in these cells. AU, absorbance units.