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Effect of anti-IgE Fabs on IgE-Fc conformation measured by FRET. The FRET ratio (E520/E485) was measured in the presence of different concentrations of anti-IgE Fabs, either FabXol3 (magenta), aϵFab (green), or control Fab (blue). aϵFab has previously been shown to fully unbend IgE-Fc (16); the control Fab binds to the Cϵ2 domain of IgE-Fc and does not cause unbending of the molecule.
