Figure 8.

Temperature dependence of the accelerated dissociation of the IgE-Fc/FabXol3 complex, mediated by the binding of the second FabXol3 molecule. Binding of the second FabXol3-binding site was characterized using SPR sandwich binding experiments at 5, 15, 25, and 35 °C (A–D). IgE-Fc was first captured on a FabXol3 surface and then a second FabXol3 molecule was added to the IgE-Fc/FabXol3 complex, in a 2-fold dilution series starting at 1.6 μm. At low temperature, almost no FabXol3-mediated accelerated dissociation of the IgE-Fc/FabXol3 complex occurs, whereas the effect is markedly increased at higher temperatures.