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Growth of M. bovis BCG Pasteur ndh mutants at 37°C. (A) Growth on solid media containing different concentrations (in micrograms per milliliter) of INH of three M. bovis BCG ndh mutants (mc22400, mc22401, and mc22402). (B) The ndh mutants and parent strain were grown in Middlebrook 7H9 broth supplemented with 10% OADC, 0.2% glycerol, and 0.05% Tween 80. The growth of the cultures was followed spectrophotometrically by measuring the OD600.
