FIG. 7.
Effect of nonpermissive temperature (42°C) on M. smegmatis ndh Ts mutants in liquid media. The ndh mutants were grown at 30°C to mid-log phase, diluted to an OD600 of ≈0.2, and shifted to 42°C (t = 0). The growth of the cultures was followed spectrophotometrically by measuring the OD600. To simplify the figures, only four mutants are shown per group. (A) Growth was ceased for the following M. smegmatis ndh Ts mutants: mc22374, mc22375, mc22376, mc22377, mc22378, mc22381, mc22383, and mc22385. (B) Shift to 42°C slows the growth of the following M. smegmatis ndh Ts mutants: mc22379, mc22380, mc22382, mc22384, mc22386, mc22387, and mc22388.