Figure 6.

SPN‐based activatable probes for fluorescence sensing of temperature. a) Chemical structures of SPs and the organic dye (RhB‐NCS) used for preparation of SPN‐based probes for temperature detection. b) Fluorescence spectra of the SPN‐P4b and SPN‐P8 probes at different temperatures. Excitation at 450 nm. c) Confocal laser microscopy images of HeLa cells incubated with the SPN‐P4b probe at 13.5 or 36.5 °C. Excitation at 458 nm. Emission at 507–518 nm was indicated in pseudo green and 571–582 nm was indicated in pseudo red. Reproduced with permission.68 Copyright 2011, American Chemical Society.