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. Author manuscript; available in PMC: 2017 Jun 16.
Published in final edited form as: J Med Chem. 2016 Apr 21;59(10):4511–4525. doi: 10.1021/acs.jmedchem.5b01762

Figure 3.

Figure 3

Compound 1 inhibits RAD51’s D-loop activity with minimal interference of RAD51-ssDNA binding. (A) Electromobility shift assay showing that 1 does not inhibit interaction with RAD51 and ssDNA. (B) Gel-based D-loop formation assay showing that 1 inhibits RAD51-mediated assimilation of a radiolabeled ssDNA oligonucleotide into the homologous region of a supercoiled dsDNA plasmid by up to 74%. Error bars indicate the standard error for four replicates with a representative gel image shown.