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. 2017 Jun 14;5:e3180. doi: 10.7717/peerj.3180

Figure 2. Gene expression profiles of passage 4 DPSC-OG (A) and DPSC-ED (B) using reverse transcriptase PCR.

Figure 2

Agarose gel electrophoresis profile of dental pulp stem cells isolated through enzymatic digestion (DPSC-ED) and outgrowth (DPSC-OG) using dental pulp stem cell markers (Cd13, Cd29, Cd105, Cd146, Cd166), and hematopoietic stem cell markers (Cd38, Cd150). Housekeeping gene Gadph was used as an internal control for the RT-PCR reaction. Cells isolated through both methods expressed mesenchymal dental pulp stem cell markers and no expression of hematopoietic stem cell markers was detected. M: 100 bp ladder.