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. 2017 Jun 14;5:e3180. doi: 10.7717/peerj.3180

Figure 7. Von Kossa staining (A) and quantification of the mineralization area (B).

Figure 7

Matrices secreted by dental pulp stem cells isolated through outgrowth (DPSC-OG) and enzymatic digestion (DPSC-ED) during osteoblastic differentiation were stained using von Kossa. The area of mineralization as depicted by the brown colour was measured and normalized to the control cell. Mineralization percentage was compared between DPSC-OG and DPSC-ED through paired T-test and p < 0.05 was considered significant (*). Values were represented by percentage of mineralization ± standard deviation.