Figure 8. FTIR spectrum of dental pulp stem cell during differentiation process to osteoblast.

Cells were cultured in control media and osteoblast differentiation medium. After 21 days, cells were harvested, freeze-dried, and analyzed as KBr pellets. The absorbance profile of DPSC-ED cultured in osteoblast differentiation medium was most similar to that of calvaria. An increase in the peak at 1,030 cm⁻¹ could be seen when compared to cells in the control medium, signifying accumulation of highly crystalline, matured apatite during osteoblastic differentiation.