Table 1. FTIR data analysis.
| Sample | Degree of bone mineralization1 | Relative amount of carbonate in bone mineral2 | Apatite maturity index3 |
|---|---|---|---|
| Calvaria | 3.696541 ± 1.122246a | 0.007106 ± 0.005761396 | 1.502549 ± 0.239476 |
| DPSC-OG (control medium) | 0 | – | 0 |
| DPSC-ED (control medium) | 0 | – | 0 |
| DPSC-OG (osteoblast differentiation medium) | 3.148611 ± 1.316780a | – | 1.279091 ± 0.065186 |
| DPSC-ED (osteoblast differentiation medium) | 7.049769 ± 1.539965b | 0.000509 ± 0.000040 | 1.124369 ± 0.094341 |
Notes.
1
Area of v1, v3 phosphate (1,200–900 cm−1): area of organic matrix amide I (1,720–1,590 cm−1).
2
Ratio of area of v2 carbonate peak (890–850 cm−1): area of phosphate peak.
3
Second derivative spectra was used to measure the ratio of peak area 1,030:1,110.
a, b
One way ANOVA was conducted to compare between calvaria and dental pulp stem cells isolated through outgrowth (DPSC-OG) and enzymatic digestion (DPSC-ED) in terms of degree of bone mineralization and apatite maturity index while paired t-test was conducted for carbonate: phosphate ratio. Values were mean ± standard deviation (n = 3) and p < 0.05 was considered as significant as denoted by different letters.