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. 2017 Aug 1;6:763. Originally published 2017 Jun 1. [Version 2] doi: 10.12688/f1000research.11577.2

Figure 6. Using the spectra acquired on MLVs and the poisson NMF pluggin in ImageJ, the acquired image stacks can be efficiently separated in as many as 4 different colours.

Figure 6.

The poisson-NMF pluggin was used to perform spectral deconvolution on the lambda-stack obtained with live cells labelled with both Laurdan and Nile red (see Figure 3). Reference curves used (lower panel) were those obtained on MLVs labelled either with Laurdan (DPPC-cholesterol (Lo): cyan curve, DOPC(Ld): orange curve) or with Nile red (DPPC-cholesterol (Lo): green curve, DOPC(Ld): red curve). Settings used were all as described in M&M. The four panels correspond to: Upper-left (cyan): Laurdan in apolar (hydrophobic) environment; Lower-left (orange): Laurdan in water-exposed environment; Upper-right (green): Nile red in apolar (hydrophobic) environment; Lower-right (red): Nile red in water-exposed environment. Laurdan Nile Red 02 from 07 April 2015.