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. 2016 Dec 16;19(4):524–534. doi: 10.1093/neuonc/now233

Fig. 4.

Fig. 4

NFκB p65 regulates NFIA transcription activity. (A) A putative NFκB p65-binding site in the human NFIA promoter luciferase reporter. (B) Luciferase activity was measured 48 h after transfection of NFIA luciferase reporter into U87 GBM cells expressing NFκB p65 siRNA (siNFκB p65) or control (siCont); n = 3; western blot of whole cell lysate of GBM cells used in the experiment B. (C) Relative luciferase activity of pGL3 wild-type (wt) or mutant (mt) NFIA promoter transfected into U87 cells expressing siNFκB p65 or siCont. (D) NFIA mRNA expression measured by RT-PCR in GBM cells transfected with NFκB p65 or vector control, n = 3. (E) ChIP-qPCR assays on GBM cells using anti-NFκB p65 or immunoglobulin G control.