Figure 6. Heatmap of the median marker intensities of the 10 lineage markers across the 20 cell populations obtained with FlowSOM after the metaclustering step with ConsensusClusterPlus (PBMC data).

The color in the heatmap represents the median of the arcsinh, 0-1 transformed marker expression calculated over cells from all the samples, varying from blue for lower expression to red for higher expression. The numbers indicate the actual expression values. The dendrogram on the left represents the hierarchical similarity between the 20 metaclusters (metric: Euclidean distance; linkage: average). Each cluster has a unique color assigned (bar on the left) which is identical in other visualizations of these 20 clusters (e.g. Figure t-SNE map in 10) facilitating the figure interpretation. Values in the brackets next to the cluster numbers indicate the relative size of clusters.