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. Author manuscript; available in PMC: 2017 Dec 7.
Published in final edited form as: Nature. 2017 Jun 7;546(7658):416–420. doi: 10.1038/nature22812

Fig.3. Simulating of human peri-gastrulation and hPGC competency.

Fig.3

a. Schematics showing progressive gain and loss of competency for hPGCs.

b. Induction of hPGCs (% NT/AP +ve cells) in day 4-5 embryoids.

c. Induction of of definitive endoderm (DE: (% CXCR4 +ve cells)) at 12h–24h of during ME differentiation.

d. Immunostaining of hPGCs induced from Pre-ME(12h) and of DE from ME(24h). Dashed lines: SOX17/OCT4 +ve hPGCs. Scale bar: 50 μm.

e. Gene expression (RT-qPCR) changes during ME induction.

f. Induction of hPGCs (%NANOS3-tdTomato/AP +ve cells) from Pre-ME(12h) or ME(24h). Pre-ME are competent for hPGCs in response cytokines or SOX17, but ME are not.

g. Immunostaining of day4 embryoids from Pre-ME(12h) or ME(24h). SOX17 in Pre-ME induced OCT4/BLIMP1 +ve hPGCs (dashed line), but in ME, it induced DE (BLIMP1/FOXA2 +ve) cells. Scale bar: 50 μm.