TABLE 2.
Expression of drug resistance genes in C. glabrata parental isolates, the first resistant mutants, and last isolates examined
| Isolate no. and type | Expression (relative to parent)a
|
||
|---|---|---|---|
| CgCDR1 | CgCDR2 | CgERG11 | |
| CBS 138 | |||
| Parent | 1 | 1 | 1 |
| Firstb | 32.1 | 27.9 | 1.7 |
| Lastc | 10.2 | 8.3 | 0.9 |
| CBS 860 | |||
| Parent | 1 | 1 | 1 |
| Firstb | 7.0 | 5.8 | 0.7 |
| Lastc | 11.7 | 8.7 | 0.4 |
| CBS 4692 | |||
| Parent | 1 | 1 | 1 |
| Firstb | 31.9 | 25.5 | 1.2 |
| Lastc | 27.6 | 14.6 | 0.5 |
| 73/124 | |||
| Parent | 1 | 1 | 1 |
| Firstb | 10.1 | 11.1 | 0.8 |
| Lastc | 6.8 | 8.7 | 0.6 |
| 75/015 | |||
| Parent | 1 | 1 | 1 |
| Firstb | 15.1 | 7.9 | 0.9 |
| Lastc | 11.6 | 8.9 | 0.6 |
a
Expression values were normalized against the amount of RNA loaded on the gel for each isolate. The ratio of gene expression after fluconazole exposure compared to gene expression by the corresponding parental isolate is shown. Values are the averages of two independent Northern hybridization experiments.
b
The first resistant mutant to emerge during fluconazole exposure.
c
The last isolate examined (exposed to fluconazole for 14 days and then cultivated in the absence of fluconazole for 122 days).