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. 2017 Jun 6;11(6):e0005657. doi: 10.1371/journal.pntd.0005657

Fig 1. LAMP-2 deficiency is enough to compromise T. cruzi invasion in host cells.

Fig 1

WT, LAMP1/2-/- or LAMP2-/- fibroblasts monolayers were exposed to Tissue Culture derived Trypomastigotes (TCT) from Y strain at a MOI of 50 for 20 minutes, washed, fixed and then processed for immunofluorescence detection of total intracellular parasites. Quantitative analysis of parasite infection rates in the three fibroblast cell lines was determined by the number of internalized parasites per 100 counted cells (A), as well as the percentage of infected cells (B). Data are shown as mean of triplicates ±SD. Asterisks indicate statistically significant differences (p<0.05, Student’s t test) between WT and LAMP deficient cells. (C) Representative panels of T. cruzi invasion in the three different fibroblast cell lines revealed by immunofluorescence labeling. Cell and parasite nuclei, as well as parasite kinetoplast DNA, were labeled with DAPI (blue); extracellular parasites in the field were labeled with anti-T. cruzi antibody followed by secondary IgG labeled with Alexa Fluor 546 (red). White arrows indicate intracellular parasites, while red arrows indicate extracellular parasites. Data shown are representative of three independent experiments.