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. 2017 Jun 16;12(6):e0179659. doi: 10.1371/journal.pone.0179659

Fig 3. Effect of phage infection on formation of Af biofilm, as studied in liquid in wells.

Fig 3

Black bars show inhibition of Af biofilm by planktonic supernatants of Pa after acute infection with phage, white bars show inhibition of Af biofilm by planktonic supernatants of the same strains of Pa cultured in the absence of phage. Grey bar shows negative control (RPMI medium only). Horizontal asterisks compare the bars at the ends of the horizontal brackets; two and three asterisks represent differences of p ≤ 0.01, p ≤ 0.001, respectively, comparing each Pa isolate uninfected vs. after phage infection. Comparison of all vs RPMI (the Af control, grey bar): ΔcoaB+ Pf4: p ≤ 0.01; PAO1+Pf4, ΔpvdD+Pf4 and ΔPAO728 + Pf4: p>0.05. All other bars: p≤0.001. Comparison PAO1 vs. its mutants: ΔpvdD and ΔPAO728: p≤0.001. All other bars: p>0.05. Comparison PAO1 vs PAK: p≤0.001.