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. 2017 Jun 16;12(6):e0179582. doi: 10.1371/journal.pone.0179582

Fig 1. Truncation or mutation of MinE’s membrane targeting sequence decreases the lower limit of the length scale of Min protein patterns.

Fig 1

(A) Confocal images of self-organized WT and Δ(2–12), L3E, L4E, F6E, F7E mutant waves on flat membranes. WT MinE and mutant proteins were titrated from 0.5 to 5 μM (MinD at 1 μM with 20% eGFP-MinD). Scale Bar: 50 μm. Dependence of the mean (B) wavelength and (C) velocity of WT and mutant waves on MinE concentration (MinD at 1 μM). Error bars represent standard deviation (N ≥ 3) from at least three independent experiments.