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(i) piRNA clusters can be transcribed if particular methylated histone (fly) or A-Myb promoter (mouse) is nearby; (ii) G-quadruplexes could have a role in piRNA processing and (iii) both first and tenth piRNA bases (respectively U and A) represent an important binding zone for Argonaute proteins, participating in a ping-pong cycle where the piRNA sequences bind with transposons.
