Fig 2. Expected OTU richness as a function of sequence depth (rarefaction).

In silico pooled replicates are shown as dashed lines and individual extraction replicates as solid lines. In panel A richness up to the maximum read number for pooled replicates and in B the same rarefaction curves up to maximum read number for individual replicates. Error bars (in B) represent standard error, for pooled samples calculated as described in Heck et al [47].