Fig 4. Rarefaction comparing Shannon diversity of pooled samples (grey lines) and individual replicates (black lines).

Expected Shannon diversity as a function of read sequence depth was estimated using repeated random sub-sampling from replicates with >15,000 reads from Fine Sand (A; n = 5), Coarse Sand (B; n = 7) and Clay (C, n = 5). Panel D shows expected richness in pooled samples compared to mean expected replicate richness.