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. 2017 Jun 6;12:4285–4297. doi: 10.2147/IJN.S131163

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© 2017 Pokrowiecki et al. This work is published and licensed by Dove Medical Press Limited

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Total AK level was congruent with the cell number (RLUs) (A) and cytotoxicity (B) in the cell culture exposed to different materials for 3 and 7 days. It can be observed that the AK levels in the cell cultures incubated in the presence of K1, K2, and the control (TCPS) significantly increased with time, which indicated cell viability and proliferation (A). The data were expressed as mean ± standard deviation (n=5). **P=0.000 between 3 and 7 days (Student’s t-test).

Notes: In the case of the Ag-containing disks, low AK levels were noticed after 3 days of incubation (A), which confirmed the increasing toxicity of these materials with incubation time (B).

Abbreviations: AK, adenylate kinase; RLU, relative luminesence unit; TCPS, tissue culture polystyrene.