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. 2017 Jun 6;12:4299–4322. doi: 10.2147/IJN.S136766

Figure 11.

Figure 11

VEGF gene expression.

Notes: (A) The expression of VEGF at the mRNA level in culture media determined by quantitative real-time PCR. (B) The expression of VEGF at the protein level in culture media detected by human VEGF ELISA kit. Both experiments were conducted for a further 72 h after transfection with Lipo/VEGF shRNA, PLGA/PPF/VEGF shRNA, or PLGA/PPF/SC shRNA. The weight ratio of nanocomposites to SC shRNA or VEGF shRNA was 150:1. *P<0.05 compared with control.

Abbreviations: PLGA, poly(d,l-lactic-co-glycolic acid); PPF, PEI-PEG-FA; PEI-PEG-FA, polyethyleneimine premodified with polyethylene glycol-folic acid; SC shRNA, scrambled small hairpin RNA; VEGF, vascular endothelial growth factor.