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. 2017 Jun 6;12:4299–4322. doi: 10.2147/IJN.S136766

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© 2017 Shen et al. This work is published and licensed by Dove Medical Press Limited

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In vitro hemolysis assay. Hemolytic activity of the LM-PLGA (A) and LM-PLGA/PPF (B) at various nanocomposite concentrations (50, 100, 200, 300, and 400 μg/mL, respectively), incubated with rat red blood cells at 37°C for 2 h. Saline and water were used as negative and positive controls, respectively. Insets on the top right are the enlarged UV-vis spectra and bottom-right insets show the photograph of red blood cells treated with LM-PLGA and LM-PLGA/PPF at various concentrations. (C) Optical microscopy images of the dispersion of erythrocytes treated with different nanoparticles. Saline was used as a control.

Abbreviations: PLGA, poly(d,l-lactic-co-glycolic acid); PPF, PEI-PEG-FA; PEI-PEG-FA, polyethyleneimine premodified with polyethylene glycol-folic acid.